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primary antibody specific to the target proteins  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary antibody specific to the target proteins
    Primary Antibody Specific To The Target Proteins, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibody specific to the target proteins/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    primary antibody specific to the target proteins - by Bioz Stars, 2026-02
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    A small group of blood vessel-like RFP cells appeared in the 7D group (A-C), which was further confirmed by the immunofluorescent staining for PDGFRβ (D), <t>CD13</t> (E), MYH11 (F), and AQP4 (G). RFP+ cells in the cortex of the 1M group (H). Blood vessel-like RFP cells in the cortex, striatum, and thalamus of the water group (I-K). Percentages of blood vessel-like RFP cells (L), PDGFRβ+ RFP cells (M), CD13+ RFP cells (N), MYH11+ RFP cells (O) in the different groups. Significance analysis using two-way analyses of variance (ANOVA) followed by Bonferroni post hoc test. Bars: 80μm for A, 20μm for B-K. The data are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. Ctx, cortex; Str, striatum; Tha, thalamus; Hth, hypothalamus; Hip, hippocampus.
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    Image Search Results


    Knockdown of OSMR inhibits activation of JAK/STAT3/CCL-2 signaling pathway in tumor cells. WB detection of JAK, p-JAK, STAT3, p-STAT3 and CCL-2 expression in cells and quantitative analysis. N = 3, ****p < 0.0001.

    Journal: Frontiers in Oncology

    Article Title: OSMR induces M2 polarization of glioblastoma associated macrophages through JAK/STAT3 signaling pathway

    doi: 10.3389/fonc.2025.1538649

    Figure Lengend Snippet: Knockdown of OSMR inhibits activation of JAK/STAT3/CCL-2 signaling pathway in tumor cells. WB detection of JAK, p-JAK, STAT3, p-STAT3 and CCL-2 expression in cells and quantitative analysis. N = 3, ****p < 0.0001.

    Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with primary antibodies specific to the target proteins: OSMR (1:1000, PA5-100298, Thermo Fisher, Massachusetts, USA), JAK (1:500, A7694, Abclonal, Wuhan, China), p-JAK (1:1000, AP0373, Abclonal, Wuhan, China), STAT3 (1:1000, AF1492, Beyotime, Shanghai, China), p-STAT3 (1:1000, GB150001, Servicebio, Wuhan, China), CCL-2 (1:1000, A21991, Abclonal, Wuhan, China), and β-actin (1:1000, ab8227, Abcam, Cambridge, UK).

    Techniques: Knockdown, Activation Assay, Expressing

    OSMR activates JAK/STAT3/CCL-2 pathway to promote malignant behavior of tumor cells. (A) WB detection of JAK, p-JAK, STAT3, p-STAT3 and CCL-2 expression in cells and quantitative analysis; (B) CCK-8 detection of cell proliferation; (C) Colony formation experiment to detect cell proliferation; (D) Transwell detects cell invasion ability; (E) Scratch test to detect cell migration ability; (F-G) Flow cytometry is used to detect cell apoptosis rate (F) and cell cycle (G) . N = 3, ***p < 0.001, ****p < 0.0001.

    Journal: Frontiers in Oncology

    Article Title: OSMR induces M2 polarization of glioblastoma associated macrophages through JAK/STAT3 signaling pathway

    doi: 10.3389/fonc.2025.1538649

    Figure Lengend Snippet: OSMR activates JAK/STAT3/CCL-2 pathway to promote malignant behavior of tumor cells. (A) WB detection of JAK, p-JAK, STAT3, p-STAT3 and CCL-2 expression in cells and quantitative analysis; (B) CCK-8 detection of cell proliferation; (C) Colony formation experiment to detect cell proliferation; (D) Transwell detects cell invasion ability; (E) Scratch test to detect cell migration ability; (F-G) Flow cytometry is used to detect cell apoptosis rate (F) and cell cycle (G) . N = 3, ***p < 0.001, ****p < 0.0001.

    Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with primary antibodies specific to the target proteins: OSMR (1:1000, PA5-100298, Thermo Fisher, Massachusetts, USA), JAK (1:500, A7694, Abclonal, Wuhan, China), p-JAK (1:1000, AP0373, Abclonal, Wuhan, China), STAT3 (1:1000, AF1492, Beyotime, Shanghai, China), p-STAT3 (1:1000, GB150001, Servicebio, Wuhan, China), CCL-2 (1:1000, A21991, Abclonal, Wuhan, China), and β-actin (1:1000, ab8227, Abcam, Cambridge, UK).

    Techniques: Expressing, CCK-8 Assay, Migration, Flow Cytometry

    OSMR/JAK/STAT3/CCL-2 pathway regulates malignant behavior of tumor cells and induces M2 polarization of macrophages. (A) RT-qPCR was used to detect the expression of M2 phenotype marker genes (CD206, CD163, IL-10) in co cultured macrophages; (B) Flow cytometry was used to detect the percentage of M2 macrophages in co cultured macrophages. N = 3, ns p > 0.05, **p < 0.01, ****p < 0.0001.

    Journal: Frontiers in Oncology

    Article Title: OSMR induces M2 polarization of glioblastoma associated macrophages through JAK/STAT3 signaling pathway

    doi: 10.3389/fonc.2025.1538649

    Figure Lengend Snippet: OSMR/JAK/STAT3/CCL-2 pathway regulates malignant behavior of tumor cells and induces M2 polarization of macrophages. (A) RT-qPCR was used to detect the expression of M2 phenotype marker genes (CD206, CD163, IL-10) in co cultured macrophages; (B) Flow cytometry was used to detect the percentage of M2 macrophages in co cultured macrophages. N = 3, ns p > 0.05, **p < 0.01, ****p < 0.0001.

    Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with primary antibodies specific to the target proteins: OSMR (1:1000, PA5-100298, Thermo Fisher, Massachusetts, USA), JAK (1:500, A7694, Abclonal, Wuhan, China), p-JAK (1:1000, AP0373, Abclonal, Wuhan, China), STAT3 (1:1000, AF1492, Beyotime, Shanghai, China), p-STAT3 (1:1000, GB150001, Servicebio, Wuhan, China), CCL-2 (1:1000, A21991, Abclonal, Wuhan, China), and β-actin (1:1000, ab8227, Abcam, Cambridge, UK).

    Techniques: Quantitative RT-PCR, Expressing, Marker, Cell Culture, Flow Cytometry

    OSMR activates JAK/STAT3/CCL-2 pathway to promote tumor growth and M2 polarization of macrophages. (A) WB detection of JAK, p-JAK, STAT3, p-STAT3 and CCL-2 expression in cells and quantitative analysis. (B) Images, tumor volume, and weight of subcutaneous tumors in each group of mice; (C) RT-qPCR was used to detect the expression of M2 phenotype marker genes (CD206, CD163, IL-10) in macrophages in tumor tissues; (D) Flow cytometry is used to detect the percentage of M2 macrophages in tumor tissue macrophages. N = 6, ns p > 0.05, ****p < 0.0001.

    Journal: Frontiers in Oncology

    Article Title: OSMR induces M2 polarization of glioblastoma associated macrophages through JAK/STAT3 signaling pathway

    doi: 10.3389/fonc.2025.1538649

    Figure Lengend Snippet: OSMR activates JAK/STAT3/CCL-2 pathway to promote tumor growth and M2 polarization of macrophages. (A) WB detection of JAK, p-JAK, STAT3, p-STAT3 and CCL-2 expression in cells and quantitative analysis. (B) Images, tumor volume, and weight of subcutaneous tumors in each group of mice; (C) RT-qPCR was used to detect the expression of M2 phenotype marker genes (CD206, CD163, IL-10) in macrophages in tumor tissues; (D) Flow cytometry is used to detect the percentage of M2 macrophages in tumor tissue macrophages. N = 6, ns p > 0.05, ****p < 0.0001.

    Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with primary antibodies specific to the target proteins: OSMR (1:1000, PA5-100298, Thermo Fisher, Massachusetts, USA), JAK (1:500, A7694, Abclonal, Wuhan, China), p-JAK (1:1000, AP0373, Abclonal, Wuhan, China), STAT3 (1:1000, AF1492, Beyotime, Shanghai, China), p-STAT3 (1:1000, GB150001, Servicebio, Wuhan, China), CCL-2 (1:1000, A21991, Abclonal, Wuhan, China), and β-actin (1:1000, ab8227, Abcam, Cambridge, UK).

    Techniques: Expressing, Quantitative RT-PCR, Marker, Flow Cytometry

    A small group of blood vessel-like RFP cells appeared in the 7D group (A-C), which was further confirmed by the immunofluorescent staining for PDGFRβ (D), CD13 (E), MYH11 (F), and AQP4 (G). RFP+ cells in the cortex of the 1M group (H). Blood vessel-like RFP cells in the cortex, striatum, and thalamus of the water group (I-K). Percentages of blood vessel-like RFP cells (L), PDGFRβ+ RFP cells (M), CD13+ RFP cells (N), MYH11+ RFP cells (O) in the different groups. Significance analysis using two-way analyses of variance (ANOVA) followed by Bonferroni post hoc test. Bars: 80μm for A, 20μm for B-K. The data are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. Ctx, cortex; Str, striatum; Tha, thalamus; Hth, hypothalamus; Hip, hippocampus.

    Journal: bioRxiv

    Article Title: Oligodendroglia generate vascular mural cells and neurons in the adult mouse brain

    doi: 10.1101/2023.09.12.549127

    Figure Lengend Snippet: A small group of blood vessel-like RFP cells appeared in the 7D group (A-C), which was further confirmed by the immunofluorescent staining for PDGFRβ (D), CD13 (E), MYH11 (F), and AQP4 (G). RFP+ cells in the cortex of the 1M group (H). Blood vessel-like RFP cells in the cortex, striatum, and thalamus of the water group (I-K). Percentages of blood vessel-like RFP cells (L), PDGFRβ+ RFP cells (M), CD13+ RFP cells (N), MYH11+ RFP cells (O) in the different groups. Significance analysis using two-way analyses of variance (ANOVA) followed by Bonferroni post hoc test. Bars: 80μm for A, 20μm for B-K. The data are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. Ctx, cortex; Str, striatum; Tha, thalamus; Hth, hypothalamus; Hip, hippocampus.

    Article Snippet: For immunofluorescence staining, the tissues were incubated overnight at 4°C with primary antibodies targeting specific proteins: CD13 (GTX75927, Genetex), Sox10 (AF2698, Beyotime), PDGFRβ (AF1042, R&D Systems; 14-1402-82, ThermoFisher), MYH11 (ab224804, Abcam), NG2 (ab5320, Millipore; ab129051, Abcam), aquaporin-4 (AQP4, 59678, CST), PDGFRα (558774, BD Biosciences), Ki67 (12202, CST; ab15580, Abcam), neuronal nuclei (NeuN, 266004, Synaptic Systems), microtubule-associated protein 2 (MAP2, 8707, CST), RFP (600-401-379, Rockland), Cre recombinase (MAB3120, Millipore), gamma-aminobutyric acid (GABA, A2052, Millipore), Reelin (ab312310, Abcam), the proto-oncogene c-Fos (2250, CST), doublecortin (DCX, 4604, CST), vesicular glutamate transporter 2 (VGLUT2, MAB5504, Millipore), adenomatous polyposis coli (APC, clone CC1, GTX16794, Genetex) and activating transcription factor 3 (ATF-3, HPA001562, Millipore).

    Techniques: Staining

    Representative images of Sox10-A cells in the cortex of the oil group (A), the oil/ethanol group (B), and the high-dose group (C). Percentages of Sox10-A cells (D), CD13+ Sox10-A cells (E), MYH11+ Sox10-A cells (F) in the three groups. Significance analysis using two-way analyses of variance (ANOVA) followed by Bonferroni post hoc test. Bar = 20μm. The data are represented as mean ± SEM. *** p < 0.001. Ctx, cortex; Str, striatum; Tha, thalamus; Hth, hypothalamus; Hip, hippocampus; HD, high dose.

    Journal: bioRxiv

    Article Title: Oligodendroglia generate vascular mural cells and neurons in the adult mouse brain

    doi: 10.1101/2023.09.12.549127

    Figure Lengend Snippet: Representative images of Sox10-A cells in the cortex of the oil group (A), the oil/ethanol group (B), and the high-dose group (C). Percentages of Sox10-A cells (D), CD13+ Sox10-A cells (E), MYH11+ Sox10-A cells (F) in the three groups. Significance analysis using two-way analyses of variance (ANOVA) followed by Bonferroni post hoc test. Bar = 20μm. The data are represented as mean ± SEM. *** p < 0.001. Ctx, cortex; Str, striatum; Tha, thalamus; Hth, hypothalamus; Hip, hippocampus; HD, high dose.

    Article Snippet: For immunofluorescence staining, the tissues were incubated overnight at 4°C with primary antibodies targeting specific proteins: CD13 (GTX75927, Genetex), Sox10 (AF2698, Beyotime), PDGFRβ (AF1042, R&D Systems; 14-1402-82, ThermoFisher), MYH11 (ab224804, Abcam), NG2 (ab5320, Millipore; ab129051, Abcam), aquaporin-4 (AQP4, 59678, CST), PDGFRα (558774, BD Biosciences), Ki67 (12202, CST; ab15580, Abcam), neuronal nuclei (NeuN, 266004, Synaptic Systems), microtubule-associated protein 2 (MAP2, 8707, CST), RFP (600-401-379, Rockland), Cre recombinase (MAB3120, Millipore), gamma-aminobutyric acid (GABA, A2052, Millipore), Reelin (ab312310, Abcam), the proto-oncogene c-Fos (2250, CST), doublecortin (DCX, 4604, CST), vesicular glutamate transporter 2 (VGLUT2, MAB5504, Millipore), adenomatous polyposis coli (APC, clone CC1, GTX16794, Genetex) and activating transcription factor 3 (ATF-3, HPA001562, Millipore).

    Techniques: